1NLF
Crystal Structure of DNA Helicase RepA in complex with sulfate at 1.95 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-05-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.83432 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 192.300, 55.300, 105.600 |
| Unit cell angles | 90.00, 122.90, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.950 |
| R-factor | 0.212 |
| Rwork | 0.201 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.230 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 1.950 | |
| Rmerge | 0.078 | 0.097 * |
| Total number of observations | 243785 * | |
| Number of reflections | 72794 | |
| <I/σ(I)> | 9.1 | |
| Completeness [%] | 98.4 | 99.6 * |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 * | 277 * | PEG 4000, TRIS buffer, magnesium sulfate , pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 17 (mg/ml) | |
| 2 | 1 | drop | MES/NaOH | 40 (mM) | pH5.8 |
| 3 | 1 | drop | 10 (mM) | ||
| 4 | 1 | drop | 80 (mM) | ||
| 5 | 1 | drop | AMP-PNP | 1 (mM) | |
| 6 | 1 | drop | (dT)12 | 100000 (mM) | |
| 7 | 1 | reservoir | 200 (mM) | ||
| 8 | 1 | reservoir | Tris-Cl | 100 (mM) | pH8.0 |
| 9 | 1 | reservoir | PEG4000 | 28 (%) |
