1NLD

FAB FRAGMENT OF A NEUTRALIZING ANTIBODY DIRECTED AGAINST AN EPITOPE OF GP41 FROM HIV-1

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1992
Detector	SIEMENS
Spacegroup name	P 21 21 21
Unit cell lengths	38.150, 82.550, 131.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.900
R-factor	0.198
Rwork	0.198
R-free	0.32600
RMSD bond length	0.008
RMSD bond angle	1.700 *
Data reduction software	XENGEN
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	5.240 *
High resolution limit [Å]	2.880 *
Rmerge	0.102
Total number of observations	27644 *
Number of reflections	8958
Completeness [%]	91.6
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		291 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	10-25 (%(w/v))
2	1	reservoir	MES	200 (mM)