1NKW

Crystal Structure Of The Large Ribosomal Subunit From Deinococcus Radiodurans

Replaces:  1LNRReplaces:  1KC9Replaces:  1KPJ

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	90
Detector technology	CCD
Collection date	2001-04-01
Detector	ADSC QUANTUM 4
Spacegroup name	I 2 2 2
Unit cell lengths	170.000, 410.000, 697.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 3.100
Rwork	0.240
R-free	0.27400
Structure solution method	COMBINATION OF MR AND MIR
Starting model (for MR)	1ffk
RMSD bond length	0.004
RMSD bond angle	0.710
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.050
High resolution limit [Å]	3.000	3.000
Rmerge	0.145 *	0.440 *
Total number of observations	4787954 *
Number of reflections	441570
Completeness [%]	95.0	48.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.8 *	18 *	ETHANOL, DIMETHYLHEXANEDIOL, MGCL2, KCL, HEPES, NH4CL, VAPOR DIFFUSION, SITTING DROP

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	ETHANOL
10	1	2	KCL
11	1	2	NH4CL
2	1	1	DIMETHYLHEXANEDIOL
3	1	1	MGCL2
4	1	1	KCL
5	1	1	NH4CL
6	1	1	HEPES
7	1	2	ETHANOL
8	1	2	DIMETHYLHEXANEDIOL
9	1	2	MGCL2

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir		10 (mM)
2	1	reservoir		60 (mM)
3	1	reservoir		5 (mM)
4	1	reservoir	HEPES	10 (mM)	pH7.8