1NKQ

Crystal structure of yeast ynq8, a fumarylacetoacetate hydrolase family protein

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Collection date	2002-10-01
Detector	BRANDEIS - B4
Wavelength(s)	0.9785
Spacegroup name	P 21 21 21
Unit cell lengths	57.705, 85.990, 316.704
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.200
Rwork	0.216
R-free	0.26200
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.400
Data reduction software	DENZO (2000)
Data scaling software	SCALEPACK (2000)
Phasing software	SnB
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.100
High resolution limit [Å]	2.000	2.000
Rmerge	0.166	0.484
Number of reflections	78576
<I/σ(I)>	6.1
Completeness [%]	73.3	30.9
Redundancy	12	12

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	4.5	293	PEG 4000, CaCl2, Ammonium Sulfate, pH 4.5, EVAPORATION, temperature 293K