1NKP
Crystal structure of Myc-Max recognizing DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Detector technology | CCD |
| Wavelength(s) | 0.91 |
| Spacegroup name | P 1 |
| Unit cell lengths | 39.244, 45.128, 86.484 |
| Unit cell angles | 87.91, 84.61, 71.50 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.219 |
| Rwork | 0.219 |
| R-free | 0.26300 |
| Structure solution method | Phased translation search |
| Starting model (for MR) | 1an2 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.950 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 1.800 | |
| Rmerge | 0.053 | |
| Total number of observations | 276421 * | |
| Number of reflections | 49228 | |
| Completeness [%] | 95.9 | 90.9 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 * | 15 * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | 3-7% PEG 4000 | ||
| 2 | 1 | 1 | 30% methyl pentane diol | ||
| 3 | 1 | 1 | 1MM cobalt hexamine chloride | ||
| 4 | 1 | 1 | 50MM sodium cacodylate pH 6.5 | ||
| 5 | 1 | 2 | PEG 4000 | ||
| 6 | 1 | 2 | methyl pentane diol | ||
| 7 | 1 | 2 | cobalt hexamine chloride | ||
| 8 | 1 | 2 | sodium cacodylate pH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 3-7 (%) | |
| 2 | 1 | reservoir | MPD | 30 (%) | |
| 3 | 1 | reservoir | cobalt hexammine chloride | 1 (mM) | |
| 4 | 1 | reservoir | sodium cacodylate | 50 (mM) | pH6.5 |
| 5 | 1 | drop | protein | 10 (mg/ml) |
