1NK1

NK1 FRAGMENT OF HUMAN HEPATOCYTE GROWTH FACTOR/SCATTER FACTOR (HGF/SF) AT 2.5 ANGSTROM RESOLUTION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.5
Synchrotron site	SRS
Beamline	PX9.5
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-08-26
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	53.420, 63.560, 56.810
Unit cell angles	90.00, 96.04, 90.00

Refinement procedure

Resolution	20.000 - 2.500
R-factor	0.245
Rwork	0.245
R-free	0.31900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 1PKR AND 2HGF
RMSD bond length	0.010
RMSD bond angle	1.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.560
High resolution limit [Å]	2.500	2.500
Rmerge	0.042 *	0.262 *
Total number of observations	44611 *
Number of reflections	13148
<I/σ(I)>	17
Completeness [%]	99.4	97.9
Redundancy	3.4	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5 *	25 *	18% PEG4000, 0.20M SODIUM ACETATE, 0.15M TRIS, PH 8.5 , pH 8.7

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	18 (%)
2	1	reservoir	sodium acetate	200 (mM)
3	1	reservoir	Tris-Cl	150 (mM)