1NJF
Nucleotide bound form of an isolated E. coli clamp loader gamma subunit
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-01-31 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.24345 , 1.28201, 1.28243 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.708, 113.347, 121.355 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 82.840 - 2.300 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.25000 * |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.347 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.350 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.053 | 0.240 |
| Total number of observations | 1061344 * | |
| Number of reflections | 45447 * | |
| <I/σ(I)> | 34.5 | 8.7 |
| Completeness [%] | 96.5 * | 84.4 |
| Redundancy | 10.4 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 2.8 | 293 | PEG 10000, KNO3, 1,4-Butanediol, pH 2.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 1 (mM) | |
| 2 | 1 | drop | AMP-PNP | 12 (mM) | |
| 3 | 1 | drop | 27 (mM) | ||
| 4 | 1 | reservoir | PEG3350 | 12-19 (%) | |
| 5 | 1 | reservoir | ammonium sulfate | 0.12-0.19 (M) |
