1NJF

Nucleotide bound form of an isolated E. coli clamp loader gamma subunit

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-01-31
Detector	ADSC QUANTUM 4
Wavelength(s)	1.24345 , 1.28201, 1.28243
Spacegroup name	P 21 21 21
Unit cell lengths	71.708, 113.347, 121.355
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	82.840 - 2.300
R-factor	0.21
Rwork	0.210
R-free	0.25000 *
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.347 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.350
High resolution limit [Å]	2.300	2.300
Rmerge	0.053	0.240
Total number of observations	1061344 *
Number of reflections	45447 *
<I/σ(I)>	34.5	8.7
Completeness [%]	96.5 *	84.4
Redundancy	10.4	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	2.8	293	PEG 10000, KNO3, 1,4-Butanediol, pH 2.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	1 (mM)
2	1	drop	AMP-PNP	12 (mM)
3	1	drop		27 (mM)
4	1	reservoir	PEG3350	12-19 (%)
5	1	reservoir	ammonium sulfate	0.12-0.19 (M)