1NI6
Comparisions of the Heme-Free and-Bound Crystal Structures of Human Heme Oxygenase-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 119 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-08-17 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.211, 55.507, 108.001 |
| Unit cell angles | 90.00, 98.88, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| Rwork | 0.217 |
| R-free | 0.26710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1QQ8 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.132 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.068 | 0.559 |
| Number of reflections | 49421 | |
| <I/σ(I)> | 20.6 | 2.02 |
| Completeness [%] | 94.0 | 87.4 |
| Redundancy | 9.5 | 4.67 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 301 | ammonium sulphate, 1,6 hexane diol, HEPES, water, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 301K |
