1NI5
Structure of the MesJ PP-ATPase from Escherichia Coli
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-10-30 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9788 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 67.508, 67.508, 204.678 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.490 - 2.650 |
| R-factor | 0.223 |
| Rwork | 0.223 |
| R-free | 0.29300 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.740 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.059 | 0.296 |
| Number of reflections | 16208 | |
| <I/σ(I)> | 20.4 | 3.5 |
| Completeness [%] | 98.7 | 96.4 |
| Redundancy | 10 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 1.5M MgFormate, pH unbuffered, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
