1NI3

Structure of the Schizosaccharomyces pombe YchF GTPase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9A
Synchrotron site	NSLS
Beamline	X9A
Temperature [K]	100
Detector technology	CCD
Collection date	2001-11-15
Detector	MARRESEARCH
Wavelength(s)	0.9790
Spacegroup name	P 65
Unit cell lengths	130.241, 130.241, 66.229
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.760 - 2.800
R-factor	0.213
Rwork	0.213
R-free	0.26500
Structure solution method	single wavelength
RMSD bond length	0.008
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.760	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.062	0.329
Number of reflections	15709
<I/σ(I)>	16.9	3.5
Completeness [%]	98.8	95.7
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	291	15% PEG 4000, 0.1M Sodium, Citrate pH 5.6, 0.1M Ammonium, Sulfate, 5%, Glycerol, Water , VAPOR DIFFUSION, HANGING DROP, temperature 291K