1NHL

SNAP-23N Structure

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Collection date	2002-09-02
Detector	ADSC QUANTUM 4
Spacegroup name	P 4 21 2
Unit cell lengths	34.905, 34.905, 81.691
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.300
R-factor	0.277
Rwork	0.273
R-free	0.31600
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	1.000
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.300
Rmerge	0.044 *
Number of reflections	1801 *
Completeness [%]	91.7 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	23 *	HEPES, CALCIUM CHLORIDE, PEG 400, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	1-4 (mg/ml)
2	1	drop	Tris-HCl	50 (mM)	pH7.5
3	1	drop		100 (mM)
4	1	reservoir	HEPES	0.1 (M)	pH7.5
5	1	reservoir		0.2 (M)
6	1	reservoir	PEG400	28 (%)