1NHH

Crystal structure of N-terminal 40KD MutL protein (LN40) complex with ADPnP and one Rubidium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	98
Detector technology	IMAGE PLATE
Collection date	2002-03-05
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	I 2 2 2
Unit cell lengths	61.747, 71.171, 189.615
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.400
R-factor	0.20161
Rwork	0.202
R-free	0.24900 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1b63
RMSD bond length	0.005
RMSD bond angle	1.126 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.340
High resolution limit [Å]	2.400 *	2.400 *
Rmerge	0.061 *	0.286 *
Number of reflections	29493
Completeness [%]	93.3 *	92.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	8.5 *	20 *	Ban, C., (1999) Cell(Cambridge,Mass.), 97, 85. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6 (mg/ml)
10	1	reservoir	PEG4000	18 (%)
2	1	drop	Tris-HCl	20 (mM)
3	1	drop	dithiothreitol	1 (mM)
4	1	drop	EDTA	1.0 (mM)
5	1	drop		150 (mM)
6	1	drop		5 (mM)
7	1	reservoir	Tris-HCl	100 (mM)
8	1	reservoir		200 (mM)
9	1	reservoir	dithiothreitol	2 (mM)