1NH7

ATP PHOSPHORIBOSYLTRANSFERASE (ATP-PRTASE) FROM MYCOBACTERIUM TUBERCULOSIS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2000-11-30
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	H 3 2
Unit cell lengths	132.540, 132.540, 110.500
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.000 * - 2.700
R-factor	0.19559
Rwork	0.192
R-free	0.26100 *
Structure solution method	MAD
RMSD bond length	0.048
RMSD bond angle	3.600 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.300	2.430
High resolution limit [Å]	2.700 *	2.700 *
Rmerge	0.067 *	0.150 *
Number of reflections	16645
Completeness [%]	99.1 *	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	16 *	pH 6.5, VAPOR DIFFUSION, HANGING DROP
1	VAPOR DIFFUSION, HANGING DROP	6.5	16 *	pH 6.5, VAPOR DIFFUSION, HANGING DROP
1	VAPOR DIFFUSION, HANGING DROP	6.5	16 *	pH 6.5, VAPOR DIFFUSION, HANGING DROP

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	MES	0.1 (M)	pH6.5
3	1	reservoir	magnesium sulfate	1.8 (M)