1NGZ
Chimeric Germline Fab 7g12-apo
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-D |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.874, 44.416, 57.863 |
| Unit cell angles | 84.22, 77.04, 89.39 |
Refinement procedure
| Resolution | 20.000 * - 1.600 |
| Rwork | 0.207 |
| R-free | 0.24100 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 * | |
| High resolution limit [Å] | 1.600 | |
| Rmerge | 0.034 * | 0.112 * |
| Total number of observations | 97524 * | |
| Number of reflections | 44964 | |
| Completeness [%] | 91.2 * | 81.9 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 * | 4 * | PEG2000 MME, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | PEG2000 MME | 22 (%) | |
| 2 | 1 | drop | 200 (mM) | ||
| 3 | 1 | drop | sodium citrate | 100 (mM) | pH4.0 |
| 4 | 1 | reservoir | PEG2000 MME | 24 (%) | |
| 5 | 1 | reservoir | ammonium acetate | 200 (mM) | |
| 6 | 1 | reservoir | cadmium sulfate | 10 (mM) | |
| 7 | 1 | reservoir | NMP | 1 (mM) | |
| 8 | 1 | reservoir | sodium acetate | 100 (mM) | pH4.2 |
