1NGY

Chimeric Mature Fab 7g12-Apo

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-D
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Spacegroup name	C 2 2 21
Unit cell lengths	60.792, 95.008, 155.362
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 2.200
Rwork	0.235
R-free	0.27300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *
High resolution limit [Å]	2.200
Rmerge	0.064 *	0.209 *
Total number of observations	200436 *
Number of reflections	22861 *
Completeness [%]	98.3 *	97.2 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.6 *	25 *	PEG 2000MME, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	PEG2000 MME	27 (%)
2	1	drop	ammonium sulfate	250 (mM)
3	1	drop	MP	0.5 (mM)
4	1	drop	HEPES	10 (mM)	pH6.6
5	1	reservoir	PEG2000MME	27 (%)
6	1	reservoir	ammonium sulfate	200 (mM)
7	1	reservoir	Tris	100 (mM)	pH8.6