1NGY
Chimeric Mature Fab 7g12-Apo
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-D |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IV |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 60.792, 95.008, 155.362 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 * - 2.200 |
Rwork | 0.235 |
R-free | 0.27300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 * | |
High resolution limit [Å] | 2.200 | |
Rmerge | 0.064 * | 0.209 * |
Total number of observations | 200436 * | |
Number of reflections | 22861 * | |
Completeness [%] | 98.3 * | 97.2 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.6 * | 25 * | PEG 2000MME, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | PEG2000 MME | 27 (%) | |
2 | 1 | drop | ammonium sulfate | 250 (mM) | |
3 | 1 | drop | MP | 0.5 (mM) | |
4 | 1 | drop | HEPES | 10 (mM) | pH6.6 |
5 | 1 | reservoir | PEG2000MME | 27 (%) | |
6 | 1 | reservoir | ammonium sulfate | 200 (mM) | |
7 | 1 | reservoir | Tris | 100 (mM) | pH8.6 |