1NGP

N1G9 (IGG1-LAMBDA) FAB FRAGMENT COMPLEXED WITH (4-HYDROXY-3-NITROPHENYL) ACETATE

Experimental procedure

Source type	SYNCHROTRON
Source details	PHOTON FACTORY
Synchrotron site	Photon Factory
Detector technology	IMAGE PLATE
Collection date	1994-02-22
Detector	SATOW
Spacegroup name	P 41 21 2
Unit cell lengths	110.691, 110.691, 98.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.400
R-factor	0.196 *
Rwork	0.196
R-free	0.25000
RMSD bond length	0.015
RMSD bond angle	0.039
Data reduction software	OSC
Phasing software	X-PLOR (2.1)
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000 *
High resolution limit [Å]	2.400	2.400 *
Rmerge	0.066
Total number of observations	76319 *
Number of reflections	21761
Completeness [%]	89.0 *	68 *
Redundancy	3.51

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5 *	10 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium salfate	1.8-1.9 (M)
2	1	reservoir	Tris	0.1 (M)
3	1	drop	Fab solution	10 (mg/ml)
4	1	drop	ammonium salfate	1.8-1.9 (M)
5	1	drop	Tris	0.1 (M)