1NG6
Structure of Cytosolic Protein of Unknown Function YqeY from Bacillus subtilis
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-12-15 |
| Detector | SBC-2 |
| Wavelength(s) | 0.9795,0.9798,0.94656 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.463, 45.820, 44.361 |
| Unit cell angles | 90.00, 101.85, 90.00 |
Refinement procedure
| Resolution | 34.710 - 1.400 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.24700 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.100 |
| Data reduction software | d*TREK |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.088 | 0.428 |
| Number of reflections | 27414 | |
| <I/σ(I)> | 30 | 2.4 |
| Completeness [%] | 99.3 | 95.7 |
| Redundancy | 6.95 | 4.44 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 19%PEG3350, 5% PEG400, 0.1M Bis Tris, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
