1NG2
Structure of autoinhibited p47phox
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-06-09 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.978, 0.9798, 0.9793, 0.95 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 99.986, 99.986, 44.875 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.700 |
| R-factor | 0.208 |
| Rwork | 0.206 |
| R-free | 0.23300 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.780 |
| High resolution limit [Å] | 1.700 | 1.701 |
| Rmerge | 0.067 | 0.439 |
| Number of reflections | 25449 | |
| <I/σ(I)> | 19.5 | 3.1 |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 4.9 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 18 * | PEG 3000, sodium citrate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20-35 (mg/ml) | |
| 2 | 1 | drop | HEPES | 20 (mM) | pH7.0 |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 2 (mM) | |
| 5 | 1 | reservoir | PEG3000 | 12 (%) | |
| 6 | 1 | reservoir | sodium citrate | pH5.0 |
