1NG0

The three-dimensional structure of Cocksfoot mottle virus at 2.7A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2002-07-14
Detector	ADSC QUANTUM 4
Wavelength(s)	0.95
Spacegroup name	P 21 21 21
Unit cell lengths	291.069, 537.250, 555.158
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.700
R-factor	0.281
Rwork	0.281
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	rice yellow mottle virus (1F2N)
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	GLRF
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.820
High resolution limit [Å]	2.700	2.700
Rmerge	0.190	0.990 *
Number of reflections	1653761 *	153029 *
Completeness [%]	79.0	70

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	3.3	296	0.25 M sodium succinate, 0.5 % PEG 8000, pH 3.3, VAPOR DIFFUSION, HANGING DROP, temperature 296K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	sodium succinate	0.25 (M)	pH3.3
3	1	reservoir	PEG8000	0.5 (%)