1NFY
CRYSTAL STRUCTURE OF HUMAN COAGULATION FACTOR XA COMPLEXED WITH RPR200095
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LURE BEAMLINE DW32 |
Synchrotron site | LURE |
Beamline | DW32 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-08-01 |
Detector | MARRESEARCH |
Wavelength(s) | 0.933 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.783, 71.828, 79.045 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.100 |
R-factor | 0.203 |
Rwork | 0.203 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.290 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR (98.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | |
High resolution limit [Å] | 2.100 | |
Rmerge | 0.058 | 0.284 * |
Number of reflections | 17692 | |
Completeness [%] | 93.5 | 96.2 * |
Redundancy | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 * | 19 * | 18-20% PEG 600, 50MM MES-NAOH,1MM RPR200095, pH 5.70, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 8 (mg/ml) | |
2 | 1 | drop | MES/NaOH | 5 (mM) | pH6.0 |
3 | 1 | drop | 5 (mM) | ||
4 | 1 | drop | compound 5 | 1000 (nM) | |
5 | 1 | reservoir | PEG600 | 18-20 (%) | |
6 | 1 | reservoir | MES/NaOH | 50 (mM) | pH5.7 |