1NFT

OVOTRANSFERRIN, N-TERMINAL LOBE, IRON LOADED OPEN FORM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	MACSCIENCE M18X
Temperature [K]	293
Detector technology	AREA DETECTOR
Collection date	1997-10-15
Detector	SIEMENS
Spacegroup name	P 63 2 2
Unit cell lengths	125.290, 125.290, 87.530
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	7.000 - 2.100
R-factor	0.189
Rwork	0.189
R-free	0.25600
Structure solution method	OTHER
RMSD bond length	0.014
RMSD bond angle	25.000 *
Data reduction software	SAINT
Data scaling software	SAINT
Phasing software	X-PLOR
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.090
Rmerge	0.079 *
Total number of observations	188024 *
Number of reflections	24284 *
Completeness [%]	100.0
Redundancy	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6 *	20 *	pH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	44.4 (mg/ml)
2	1	drop	Bis Tris-HCl	0.05 (M)
3	1	reservoir	Bis-Tris	0.05 (M)
4	1	reservoir	ammonium sulfate	52 (%)