1NFP

STRUCTURAL REFINEMENT OF THE NON-FLUORESCENT FLAVOPROTEIN FROM PHOTOBACTERIUM LEIOGNATHI AT 1.60 ANGSTROMS RESOLUTION

Experimental procedure

Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Detector technology	IMAGE PLATE
Collection date	1992-11
Detector	FUJI
Wavelength(s)	1.00
Spacegroup name	C 2 2 21
Unit cell lengths	56.930, 92.230, 99.230
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.600
R-factor	0.175
RMSD bond length	0.015
RMSD bond angle	0.036
Data reduction software	WEIS
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	1.600 *	1.600 *
Rmerge	0.059
Total number of observations	84138 *
Number of reflections	28223	2590 *
Completeness [%]	81.0	65.6 *
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5-10 (mg/ml)
2	1	drop	ammonium sulfate	30-34 (%sat)
3	1	drop	sodium citrate	50 (mM)
4	1	drop	isopropanol	3 (%(w/v))