1NE4
Crystal Structure of Rp-cAMP Binding R1a Subunit of cAMP-dependent Protein Kinase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 200 |
Detector technology | IMAGE PLATE |
Collection date | 2001-06-05 |
Detector | MARRESEARCH |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 86.900, 86.900, 179.000 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.400 |
Rwork | 0.209 |
R-free | 0.25300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rgs |
RMSD bond length | 0.007 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.048 * | 0.473 * |
Number of reflections | 16251 * | |
<I/σ(I)> | 47.9 | 6.3 |
Completeness [%] | 99.3 | 97.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 22.5 * | amino sulfate, glycerol, DTT, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.5K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 12 (mg/ml) | |
2 | 1 | reservoir | 1.0 (M) | ||
3 | 1 | reservoir | glycerol | 12.5 (%) | |
4 | 1 | reservoir | dithiothreitol | 10 (mM) | |
5 | 1 | reservoir | 0.1 (M) | pH5.5 |