1NE2
Crystal Structure of Thermoplasma acidophilum 1320 (APC5513)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-09-01 |
| Detector | SBC-2 |
| Wavelength(s) | 0.97905, 0.97924, 0.9372 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.086, 53.359, 149.807 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.470 - 1.750 |
| R-factor | 0.209 |
| Rwork | 0.209 |
| R-free | 0.23600 |
| Structure solution method | autoSHARP |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | d*TREK |
| Phasing software | autoSHARP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.470 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.047 | 0.369 |
| Number of reflections | 40030 | |
| <I/σ(I)> | 7.7 | 3 |
| Completeness [%] | 98.6 | 97.9 |
| Redundancy | 6.5 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | sodium acetate, PEG 4000, Glycerol, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | sodium acetate, PEG 4000, Glycerol, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
