1NDN
MOLECULAR STRUCTURE OF NICKED DNA. MODEL T4
Experimental procedure
| Source type | ROTATING ANODE |
| Temperature [K] | 298 |
| Detector technology | DIFFRACTOMETER |
| Detector | RIGAKU AFC-5R |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 25.990, 44.030, 66.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | ? - 3.000 |
| R-factor | 0.186 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Phasing software | ULTIMA |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 3.000 |
| Number of reflections | 553 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 5 | 4 * | pH 5.00, VAPOR DIFFUSION, temperature 277.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | MGCL2 | ||
| 4 | 1 | 1 | NA CACODYLATE | ||
| 5 | 1 | 1 | SPERMINE_HCL | ||
| 6 | 1 | 2 | WATER | ||
| 7 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | oligonucleotide | 0.5 (mM) | ||
| 3 | 1 | sodium cacodylate | 29 (mM) | ||
| 4 | 1 | spermine-4HCL | 8.6 (mM) | ||
| 5 | 1 | Hoechest33258 | 1.9 (mM) | ||
| 6 | 1 | 1 | MPD | 2 (%) |
