1NCN

the receptor-binding domain of human B7-2

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9B
Synchrotron site	NSLS
Beamline	X9B
Temperature [K]	100
Detector technology	CCD
Collection date	1999-01-01
Detector	ADSC QUANTUM 4
Wavelength(s)	0.96110, 0.98000, 0.98019
Spacegroup name	P 21 21 2
Unit cell lengths	56.690, 63.010, 58.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 2.700
R-factor	0.252
Rwork	0.214
R-free	0.27800
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.095 *	0.319 *
Total number of observations	44045 *
Number of reflections	11122 *	1110 *
Completeness [%]	99.8 *	100 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	4 *	Zhang, X., (2002) PROTEIN EXPRESSION PURIF., 25, 105. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir		0.1 (M)
2	1	reservoir	Tris-HCl	0.1 (M)
3	1	reservoir	PEG4000	16-26 (%)	pH8.5