1NCL

THERMAL STABILITY OF HEXAMERIC AND TETRAMERIC NUCLEOSIDE, DIPHOSPHATE KINASES

Experimental procedure

Source type	SYNCHROTRON
Source details	LURE
Synchrotron site	LURE
Collection date	1995-05
Detector	MARRESEARCH
Spacegroup name	P 63 2 2
Unit cell lengths	74.300, 74.300, 105.300
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 2.200
R-factor	0.181
Rwork	0.181
RMSD bond length	0.015
RMSD bond angle	1.680
Data reduction software	MOSFLM
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.200 *
Rmerge	0.063
Total number of observations	22178 *
Number of reflections	8556 *
Completeness [%]	93.0 *
Redundancy	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	PEG6000	5.5 (%)
3	1	drop		20 (mM)
4	1	drop	Tris-HCl	50 (mM)
5	1	reservoir	PEG6000	11 (%)
6	1	reservoir	Tris-HCl	50 (mM)