1NC9

STREPTAVIDIN MUTANT Y43A WITH IMINOBIOTIN AT 1.8A RESOLUTION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1998-02-13
Detector	RIGAKU RAXIS II
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	58.400, 87.300, 47.100
Unit cell angles	90.00, 99.00, 90.00

Refinement procedure

Resolution	10.000 - 1.800
R-factor	0.2008
Rwork	0.199
R-free	0.26600
Structure solution method	isomorphous
Starting model (for MR)	1swa
RMSD bond length	0.006
RMSD bond angle	0.024 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.800
Rmerge	0.090 *	0.790 *
Number of reflections	34344
Completeness [%]	79.0 *	28 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.5	293	MPD, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20-30 (mg/ml)
2	1	reservoir	MPD	50 (%)