1NC5

Structure of Protein of Unknown Function of YteR from Bacillus Subtilis

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2002-11-12
Detector	SBC-2
Wavelength(s)	0.9795,0.9798,0.94656
Spacegroup name	P 61 2 2
Unit cell lengths	91.855, 91.855, 179.410
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.490 - 1.600
R-factor	0.187
Rwork	0.187
R-free	0.21400
Structure solution method	MAD
Starting model (for MR)	none
RMSD bond length	0.007
RMSD bond angle	20.300 *
Data reduction software	HKL-2000
Data scaling software	d*TREK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.102	0.560 *
Number of reflections	59188 *
<I/σ(I)>	34.88	2.47
Completeness [%]	99.3	98.3
Redundancy	20.36	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295 *	1.3 M Nacitrate, 0.1M Tris , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	30 (mg/ml)
2	1	reservoir	sodium citrate	1.3 (M)
3	1	reservoir	Tris	0.1 (M)	pH7.5