1NC5
Structure of Protein of Unknown Function of YteR from Bacillus Subtilis
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-12 |
| Detector | SBC-2 |
| Wavelength(s) | 0.9795,0.9798,0.94656 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 91.855, 91.855, 179.410 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.490 - 1.600 |
| R-factor | 0.187 |
| Rwork | 0.187 |
| R-free | 0.21400 |
| Structure solution method | MAD |
| Starting model (for MR) | none |
| RMSD bond length | 0.007 |
| RMSD bond angle | 20.300 * |
| Data reduction software | HKL-2000 |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.102 | 0.560 * |
| Number of reflections | 59188 * | |
| <I/σ(I)> | 34.88 | 2.47 |
| Completeness [%] | 99.3 | 98.3 |
| Redundancy | 20.36 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 * | 1.3 M Nacitrate, 0.1M Tris , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 30 (mg/ml) | |
| 2 | 1 | reservoir | sodium citrate | 1.3 (M) | |
| 3 | 1 | reservoir | Tris | 0.1 (M) | pH7.5 |
