1NBE
ASPARTATE TRANSCARBAMOYLASE REGULATORY CHAIN MUTANT (T82A)
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 292 |
| Detector technology | AREA DETECTOR |
| Collection date | 1996-07 |
| Detector | XUONG-HAMLIN MULTIWIRE |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 122.470, 122.470, 142.770 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.600 |
| R-factor | 0.177 |
| Rwork | 0.177 |
| R-free | 0.23700 |
| Structure solution method | RIGID BODY |
| Starting model (for MR) | 6at1 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 24.800 * |
| Data reduction software | SDMS |
| Data scaling software | SDMS |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.200 | 2.700 |
| High resolution limit [Å] | 2.550 | 2.600 |
| Rmerge | 0.093 | 0.381 |
| Number of reflections | 37782 * | |
| <I/σ(I)> | 6.3 | 1.2 |
| Completeness [%] | 95.5 | 88.5 |
| Redundancy | 3.9 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 5.8 * | Ke, H.M., (1984) Proc.Natl.Acad.Sci.U.S.A., 81, 4037. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 5-10 (mg/ml) | |
| 2 | 1 | 2 | maleic acid | 100 (mM) | |
| 3 | 1 | 2 | Tris-HCl | 10 (mM) | |
| 4 | 1 | 2 | 2-mercaptoethanol | 1 (mM) | |
| 5 | 1 | 2 | EDTA | 0.2 (mM) | |
| 6 | 1 | 2 | 0.3 (mM) |
