1NBE

ASPARTATE TRANSCARBAMOYLASE REGULATORY CHAIN MUTANT (T82A)

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	292
Detector technology	AREA DETECTOR
Collection date	1996-07
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	P 3 2 1
Unit cell lengths	122.470, 122.470, 142.770
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.600
R-factor	0.177
Rwork	0.177
R-free	0.23700
Structure solution method	RIGID BODY
Starting model (for MR)	6at1
RMSD bond length	0.017
RMSD bond angle	24.800 *
Data reduction software	SDMS
Data scaling software	SDMS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.200	2.700
High resolution limit [Å]	2.550	2.600
Rmerge	0.093	0.381
Number of reflections	37782 *
<I/σ(I)>	6.3	1.2
Completeness [%]	95.5	88.5
Redundancy	3.9	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	5.8 *		Ke, H.M., (1984) Proc.Natl.Acad.Sci.U.S.A., 81, 4037. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	5-10 (mg/ml)
2	1	2	maleic acid	100 (mM)
3	1	2	Tris-HCl	10 (mM)
4	1	2	2-mercaptoethanol	1 (mM)
5	1	2	EDTA	0.2 (mM)
6	1	2		0.3 (mM)