1NBA

CRYSTAL STRUCTURE ANALYSIS, REFINEMENT AND ENZYMATIC REACTION MECHANISM OF N-CARBAMOYLSARCOSINE AMIDOHYDROLASE FROM ARTHROBACTER SP. AT 2.0 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	C 1 2 1
Unit cell lengths	136.220, 122.290, 70.870
Unit cell angles	90.00, 91.82, 90.00

Refinement procedure

Resolution	10.000 * - 2.000
R-factor	0.186
Rwork	0.186
RMSD bond length	0.010
RMSD bond angle	2.700
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	2.000 *	2.000 *
Rmerge	0.111 *
Total number of observations	147634 *
Number of reflections	62120 *
Completeness [%]	73.2 *	40.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5 *		drop-0.002ml, reservoir-0.003ml *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	26 (mg/ml)
2	1	drop	magnesium sulfate	1.25 (M)
3	1	reservoir	trimethylphosphate	20 (mM)	0.003ml