1NB7
HC-J4 RNA polymerase complexed with short RNA template strand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-09-21 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.87 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 106.660, 108.500, 134.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 * - 2.900 |
| Rwork | 0.229 |
| R-free | 0.29500 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.008 * |
| RMSD bond angle | 1.590 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 |
| High resolution limit [Å] | 2.900 |
| Rmerge | 0.135 * |
| Number of reflections | 33632 |
| <I/σ(I)> | 4.5 |
| Completeness [%] | 95.2 |
| Redundancy | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 5 | 18 * | PEG 4000, MES, Glycerol, DTT , pH 5.0, VAPOR DIFFUSION, temperature 291K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MES |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | Tris | 10 (mM) | pH7.5 | |
| 2 | 1 | 1 | 600 (mM) | ||
| 3 | 1 | 1 | glycerol | 10 (%(w/v)) | |
| 4 | 1 | 1 | dithiothreitol | 5 (mM) | |
| 5 | 1 | 1 | MES | 50 (mM) | pH5.0 |
| 6 | 1 | 1 | PEG4000 | 20 (%) | |
| 7 | 1 | 1 | glycerol | 10 (%) | |
| 8 | 1 | 1 | dithiothreitol | 50 (mM) |
