1NB4
HC-J4 RNA polymerase apo-form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-03-05 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.488 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 106.690, 108.710, 135.060 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 * - 2.000 |
| Rwork | 0.204 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1quv |
| RMSD bond length | 0.014 * |
| RMSD bond angle | 1.840 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 * | |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.103 * | |
| Number of reflections | 99357 | |
| <I/σ(I)> | 9.1 | |
| Completeness [%] | 98.6 * | |
| Redundancy | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 5 | 18 * | PEG 4000, MES, Glycerol, DTT, pH 5.0, VAPOR DIFFUSION, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | Tris | 10 (mM) | pH7.5 |
| 2 | 1 | 1 | 600 (mM) | ||
| 3 | 1 | 1 | glycerol | 10 (%(w/v)) | |
| 4 | 1 | 1 | dithiothreitol | 5 (mM) | |
| 5 | 1 | 1 | MES | 50 (mM) | pH5.0 |
| 6 | 1 | 1 | PEG4000 | 20 (%) | |
| 7 | 1 | 1 | glycerol | 10 (%) | |
| 8 | 1 | 1 | dithiothreitol | 50 (mM) |
