1NAY

GPP-Foldon:X-ray structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ELLIOTT GX-20
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	48.591, 27.901, 112.274
Unit cell angles	90.00, 105.27, 90.00

Refinement procedure

Resolution	8.000 - 2.600
R-factor	0.234
Rwork	0.234
R-free	0.29600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006 *
RMSD bond angle	1.730 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.600	2.600 *
Rmerge	0.107 *	0.224 *
Total number of observations	25421 *
Number of reflections	4410
Completeness [%]	95.1 *	95 *
Redundancy	2.2 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	PEG 4000, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7 (mg/ml)
2	1	drop	sodium acetate	0.1 (M)
3	1	drop	ammonium sulfate	0.2 (M)
4	1	drop	PEG4000	25 (%)	pH4.6
5	1	reservoir	magnesium sulfate	50-100 (mM)