1NAI

UDP-GALACTOSE 4-EPIMERASE FROM ESCHERICHIA COLI, OXIDIZED

Experimental procedure

Spacegroup name	P 32 2 1
Unit cell lengths	83.500, 83.500, 108.200
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.000
R-factor	0.193 *
Rwork	0.193
RMSD bond length	0.013
RMSD bond angle	15.600 *
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *
High resolution limit [Å]	1.800 *	1.800 *
Rmerge	0.034 *	0.225 *
Total number of observations	63946 *
Number of reflections	39504	7122 *
Completeness [%]	96.0	93 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	60 (mg/ml)
2	1	drop	UDP	5 (mM)
3	1	drop	PEG8000	15 (%)
4	1	drop		500 (mM)
5	1	drop	HEPES	50 (mM)
6	1	reservoir	PEG	15 (%)