1NA8

Crystal structure of ADP-ribosylation factor binding protein GGA1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 32 2 1
Unit cell lengths	65.426, 65.426, 142.725
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 2.300
R-factor	0.21869
Rwork	0.216
R-free	0.27900 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ID 1GYU
RMSD bond length	0.019
RMSD bond angle	1.720 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.000	2.360
High resolution limit [Å]	2.300	2.300
Rmerge	0.053	0.306
Number of reflections	16193
<I/σ(I)>	23.7	5.9
Completeness [%]	99.0	99
Redundancy	5.6	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	289	sodium citrate, ammonium sulphate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 289K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	37 (mg/ml)
2	1	reservoir	sodium citrate	100 (mM)	pH5.6
3	1	reservoir	ammonium sulfate	1.7 (M)