1NA7
Crystal structure of the catalytic subunit of human protein kinase CK2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID13 |
| Synchrotron site | ESRF |
| Beamline | ID13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9755 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.676, 45.954, 63.956 |
| Unit cell angles | 90.00, 111.82, 90.00 |
Refinement procedure
| Resolution | 29.000 * - 2.400 |
| R-factor | 0.215 |
| Rwork | 0.209 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m2r |
| RMSD bond length | 0.007 |
| RMSD bond angle | 23.500 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.000 * | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.130 | 0.370 |
| Number of reflections | 12457 | 1817 * |
| <I/σ(I)> | 4.3 | 1.6 |
| Completeness [%] | 99.0 | 99.4 |
| Redundancy | 2.3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 | 293 | PEG 3500, NaAC, Tris, pH 8, LB Modified hanging drop method, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | PEG3500 | 25 (%) | |
| 2 | 1 | drop | sodium acetate | 0.2 (M) | |
| 3 | 1 | drop | Tris | 0.1 (M) | pH8. |
| 4 | 1 | reservoir | PEG3350 | 25 (%) |
