1N9H
structure of microgravity-grown oxidized myoglobin mutant YQR (ISS6A)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-07-27 |
Detector | MARRESEARCH |
Wavelength(s) | 1.001 |
Spacegroup name | P 6 |
Unit cell lengths | 90.580, 90.580, 45.254 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 15.000 - 1.800 |
Rwork | 0.154 |
R-free | 0.15900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1f65 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.010 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 1.520 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.045 | 0.320 |
Number of reflections | 31153 * | |
<I/σ(I)> | 13.4 | 10.2 |
Completeness [%] | 98.3 | 96.7 |
Redundancy | 3.4 | 2.21 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 8.7 | 293 | ammonium sulfate, tris, EDTA, pH 8.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | reservoir | Tris-HCl | 100 (mM) | pH8.7 |
3 | 1 | reservoir | EDTA | 1 (mM) | |
4 | 1 | reservoir | ammonium sulfate | 2.8 (M) |