1N8Y
Crystal structure of the extracellular region of rat HER2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2001-02-20 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 130.870, 116.400, 55.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 2.400 |
| R-factor | 0.2237 |
| Rwork | 0.224 |
| R-free | 0.28200 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m6b |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.720 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 * | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.062 * | 0.770 * |
| Total number of observations | 196228 * | |
| Number of reflections | 34177 * | |
| <I/σ(I)> | 2 | |
| Completeness [%] | 99.2 | 93.9 |
| Redundancy | 3.7 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 298 | 15-30% PEG4000, 50mM Na citrate pH5.4, 10mM EDTA, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | Tris | 10 (mM) | pH7.5 |
| 3 | 1 | reservoir | PEG4000 | 15-30 (%) | |
| 4 | 1 | reservoir | sodium citrate | 50 (mM) | pH5.4 |
| 5 | 1 | reservoir | EDTA | 10 (mM) |
