1N8T

The crystal structure of phosphoglucose isomerase from rabbit muscle

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1996-09-27
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
Unit cell lengths	83.067, 115.174, 271.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.500
R-factor	0.181 *
Rwork	0.178
R-free	0.23400 *
Structure solution method	MIR and multi-crystal averaging
RMSD bond length	0.010
RMSD bond angle	1.440 *
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000 *	2.440
High resolution limit [Å]	2.400	2.400
Rmerge	0.055	0.184
Total number of observations	166547 *
Number of reflections	47443
<I/σ(I)>	17.7	4.8
Completeness [%]	91.6	68.8
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	294	polyethylene glycol 6000, Tris, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	reservoir	PEG6000	12-14 (%)
3	1	reservoir	Tris-HCl		pH6.8-7.5