1N8P

Crystal Structure of cystathionine gamma-lyase from yeast

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	CCD
Collection date	1998-03-15
Detector	MARRESEARCH
Wavelength(s)	1.06
Spacegroup name	C 1 2 1
Unit cell lengths	153.819, 62.164, 160.837
Unit cell angles	90.00, 105.42, 90.00

Refinement procedure

Resolution	6.000 - 2.600
Rwork	0.253
R-free	0.34600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1cs1
RMSD bond length	0.011
RMSD bond angle	1.700
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.900	2.680
High resolution limit [Å]	2.600	2.600
Rmerge	0.047	0.317
Number of reflections	44941
<I/σ(I)>	6.2	3.1
Completeness [%]	98.9	91
Redundancy		3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.2	294	18% PEG 4000, 0.2M ammonium sulfate, 10 mM phosphate buffer, pH 8.2, VAPOR DIFFUSION, SITTING DROP, temperature 294K