1N84
HUMAN SERUM TRANSFERRIN, N-LOBE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-07-26 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.015, 57.883, 135.661 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 2.050 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.22900 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a8e |
| RMSD bond length | 0.008 |
| RMSD bond angle | 22.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.077 | 0.178 |
| Number of reflections | 20284 * | 2046 * |
| <I/σ(I)> | 19.5 | 9.4 |
| Completeness [%] | 88.1 * | 91.6 |
| Redundancy | 4.4 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.7 | 20 * | 0.2M POTASSIUM ACETATE, 20% PEG 3350, pH 7.7, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 35 (mg/ml) | |
| 2 | 1 | drop | ammonium bicarbonate | 0.1 (M) | |
| 3 | 1 | reservoir | potassium acetate | 0.2 (M) | pH7.7 |
| 4 | 1 | reservoir | PEG3350 | 20 (%) |
