1N7U

THE RECEPTOR-BINDING PROTEIN P2 OF BACTERIOPHAGE PRD1: CRYSTAL FORM I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-12-13
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 2 2 21
Unit cell lengths	138.200, 46.500, 136.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.220 - 2.400
R-factor	0.224
Rwork	0.224
R-free	0.24800
Structure solution method	MAD
Starting model (for MR)	Se-Met P2
RMSD bond length	0.006
RMSD bond angle	26.400 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.000	2.550
High resolution limit [Å]	2.400	2.400
Rmerge	0.071 *	0.477 *
Number of reflections	32726
<I/σ(I)>	12
Completeness [%]	92.7	78.7 *
Redundancy	9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.6	4 *	used macroseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	MPD	30 (%)
2	1	reservoir	sodium acetate	0.1 (M)	pH4.6
3	1	reservoir		20 (mM)