1N7N
Streptococcus pneumoniae Hyaluronate Lyase W292A Mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-01-10 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 84.069, 103.584, 101.706 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 * - 1.550 |
R-factor | 0.18 |
Rwork | 0.180 |
R-free | 0.20900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1loh |
RMSD bond length | 0.015 * |
RMSD bond angle | 1.350 * |
Data reduction software | MADNESS |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.610 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.058 | 0.807 |
Total number of observations | 636169 * | |
Number of reflections | 125839 * | |
Completeness [%] | 97.3 | 87.5 |
Redundancy | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | AMMONIUM SULFATE, SODIUM CACODYLATE, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5.10 (mg/ml) | |
2 | 1 | reservoir | ammonium sulfate | 60-65 (%sat) | |
3 | 1 | reservoir | 0.2 (M) | ||
4 | 1 | reservoir | dioxane | 2 (%) | |
5 | 1 | reservoir | sodium citrate | 50 (mM) | pH6.0 |