1N7M
Germline 7G12 with N-methylmesoporphyrin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.274, 76.236, 60.435 |
| Unit cell angles | 90.00, 93.33, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 1.800 |
| Rwork | 0.220 |
| R-free | 0.26000 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 * | |
| High resolution limit [Å] | 1.800 | |
| Rmerge | 0.056 * | 0.226 * |
| Total number of observations | 125917 * | |
| Number of reflections | 17466 * | |
| Completeness [%] | 95.5 * | 67.9 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 * | 4 * | 22% PEG2000MME, 0.2M Ammonium Sulfate, 10mM Cd Sulfate, 1mM NMP, 0.1M sodium acetate, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | PEG2000 MME | 22 (%) | |
| 2 | 1 | drop | 200 (mM) | ||
| 3 | 1 | drop | sodium citrate | 100 (mM) | pH4.0 |
| 4 | 1 | reservoir | PEG2000 MME | 24 (%) | |
| 5 | 1 | reservoir | ammonium acetate | 200 (mM) | |
| 6 | 1 | reservoir | cadmium sulfate | 10 (mM) | |
| 7 | 1 | reservoir | NMP | 1 (mM) | |
| 8 | 1 | reservoir | sodium acetate | 100 (mM) | pH4.2 |
