1N7K
Unique tetrameric structure of deoxyribose phosphate aldolase from Aeropyrum pernix
Experimental procedure
| Experimental method | MIRAS |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 283 |
| Detector technology | CCD |
| Collection date | 2002-06-28 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 75.293, 84.185, 87.238 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 * - 2.000 |
| R-factor | 0.224 |
| Rwork | 0.224 |
| R-free | 0.26300 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.005 |
| RMSD bond angle | 21.700 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.500 * | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.056 * | 0.157 * |
| Number of reflections | 38099 | |
| <I/σ(I)> | 9.3 | 2 |
| Completeness [%] | 99.8 | 98.4 |
| Redundancy | 7.1 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 20 * | pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | 0.7-1.2 (M) | ||
| 3 | 1 | reservoir | 250 (mM) | ||
| 4 | 1 | reservoir | 100 (mM) | pH4.2 |
