1N7K

Unique tetrameric structure of deoxyribose phosphate aldolase from Aeropyrum pernix

Experimental procedure

Experimental method	MIRAS
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	283
Detector technology	CCD
Collection date	2002-06-28
Detector	ADSC QUANTUM 4
Wavelength(s)	1.00
Spacegroup name	P 21 21 2
Unit cell lengths	75.293, 84.185, 87.238
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 * - 2.000
R-factor	0.224
Rwork	0.224
R-free	0.26300
Structure solution method	MIRAS
RMSD bond length	0.005
RMSD bond angle	21.700 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.500 *	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.056 *	0.157 *
Number of reflections	38099
<I/σ(I)>	9.3	2
Completeness [%]	99.8	98.4
Redundancy	7.1	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.2	20 *	pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir		0.7-1.2 (M)
3	1	reservoir		250 (mM)
4	1	reservoir		100 (mM)	pH4.2