1N7B
RIP-Radiation-damage Induced Phasing
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-02-08 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.933 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 31.247, 51.154, 70.834 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.420 * |
R-factor | 0.15453 |
Rwork | 0.152 |
R-free | 0.19900 * |
Structure solution method | RIP - Radiation-damage Induced Phasing |
RMSD bond length | 0.018 |
RMSD bond angle | 1.935 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHELXD |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.480 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.095 | 0.464 * |
Number of reflections | 22939 | |
<I/σ(I)> | 10.99 | 3.1 |
Completeness [%] | 99.3 | 98.1 |
Redundancy | 4.2 * | 4.2 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 20 * | 1 mM (single strand) RNA, 20 mM sodium cacodylate buffer, pH 6.0, 5 mM spermine tetrachloride, 32 mM KCl and 2 % (v/v) methyl-2,4-pentanediol (MPD) against 25 % MPD in the reservoir, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | RNA | ||
2 | 1 | 1 | sodium cacodylate | ||
3 | 1 | 1 | spermine tetrachloride | ||
4 | 1 | 1 | KCl | ||
5 | 1 | 1 | MPD | ||
6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | 1 (mM) | single strand | |
2 | 1 | drop | 20 (mM) | pH6.0 | |
3 | 1 | drop | 5 (mM) | ||
4 | 1 | drop | 32 (mM) | ||
5 | 1 | drop | 2 (%(v/v)) | ||
6 | 1 | reservoir | 25 (%) |