1N78

Crystal structure of Thermus thermophilus glutamyl-tRNA synthetase complexed with tRNA(Glu) and glutamol-AMP.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2001-06-12
Detector	MARRESEARCH
Wavelength(s)	0.9
Spacegroup name	C 2 2 21
Unit cell lengths	110.492, 219.870, 135.119
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 * - 2.100
R-factor	0.22
Rwork	0.219
R-free	0.25900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB 1G59
RMSD bond length	0.006
RMSD bond angle	21.000 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS (1.1)
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.200
High resolution limit [Å]	2.100	2.100
Rmerge	0.074	0.342
Total number of observations	426837 *
Number of reflections	92817
Completeness [%]	96.4	88.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.5 *	4 or 20 *	PEG1500, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	PEG1500
2	1	2	PEG1500

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5.0 (mg/ml)
2	1	drop	MOPS/Na	10 (mM)	pH6.5
3	1	drop		5 (mM)
4	1	drop	2-mercaptoethanol	2.5 (mM)
5	1	drop	PEG6000	1 (%)
6	1	drop	ATP	2 (mM)
7	1	drop	glutamate	2 (mM)
8	1	reservoir	PEG	10 (%)