1N70
The Crystal Structure of Nitrite Reductase Mutant His287Ala from Rhodobacter Sphaeroides
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 200 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-05-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9836 |
| Spacegroup name | H 3 |
| Unit cell lengths | 71.395, 71.395, 146.179 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 13.250 - 1.600 |
| R-factor | 0.174 |
| Rwork | 0.174 |
| R-free | 0.20200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 13.250 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 36276 | |
| Completeness [%] | 98.8 | 99.5 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | PEG 4000, PEG 6000 or PEG 3350, MgCl2 , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
