1N6X
RIP-phasing on Bovine Trypsin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-06-13 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.209, 56.658, 66.126 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.000 * - 1.400 |
| R-factor | 0.13919 |
| Rwork | 0.138 |
| R-free | 0.16700 * |
| Structure solution method | RIP - Radiation-Damage Induced Phasing |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.626 |
| Data scaling software | SCALA |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 * | 1.470 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.047 | 0.227 |
| Number of reflections | 40997 | |
| <I/σ(I)> | 11.3 | 3.6 |
| Completeness [%] | 99.7 * | 98.3 |
| Redundancy | 13.4 | 12.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 25% polyethylene glycol (PEG) 8000, 0.2 M ammonium sulphate, 0.1 M Tris buffer, pH 8, and 100 mM benzylamine, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 25 (%) | |
| 2 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
| 3 | 1 | reservoir | Tris | 0.1 (M) | pH8. |
| 4 | 1 | reservoir | benzylamine | 100 (mM) | |
| 5 | 1 | drop | protein | 15 (mg/ml) |
